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유동세포계측법에 의해 플라즈마 막에 GLUT4 Translocation의 양적 측정
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November 07, 2010
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Summary
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이 프로토콜은 유동세포계측법에 의해 세포의 플라즈마 막으로 세포질에서 GLUT4의 translocation을 수치 빠른 방법을 설명합니다.
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Quantitative MeasurementGLUT4 TranslocationPlasma MembraneFlow CytometryGlucoseEnergy SourceBlood ConcentrationInsulin ReleaseType-2 DiabetesObesityInsulin ResistanceGlucose HomeostasisMechanismsTreatment StrategiesGLUT FamilyGlucose TransportersGLUT4 TransporterSkeletal MuscleInsulin ReceptorVesiclesCytoplasmImmunocytochemistryFluorophore-conjugated AntibodiesTechniqueQuantifyTotal Amounts Of GLUT4 Translocation
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Koshy, S., Alizadeh, P., Timchenko, L. T., Beeton, C. Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry. J. Vis. Exp. (45), e2429, doi:10.3791/2429 (2010).
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