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含量自发活动，研究昼夜节律和睡眠参数果蝇</em
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila
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含量自发活动，研究昼夜节律和睡眠参数果蝇</em

DOI:
10.3791/2157-v

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18:08 min

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September 28, 2010

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, , , ,
1Center for Advanced Biotechnology and Medicine,Rutgers University, 2Current Address: Department of Entomology, College of Agricultural and Environmental Sciences,University of California, Davis, 3Department of Molecular Biology and Biochemistry,Rutgers University

Capitoli

  • 00:00Titolo
  • 01:20Setting Up the Locomotor Activity Monitoring System
  • 03:50Preparation of Experimental Animals
  • 05:08Preparation of Activity Tubes
  • 10:03Loading Flies into Activity Tubes and Locomotor Activity Monitoring System
  • 11:36Experimental Design to Record Data for Determination of Circadian Periodicity and Amplitude
  • 13:54Representative Circadian Sleep/Rest Parameters
  • 17:47Conclusion

Summary

Traduzione automatica

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我们描述用于记录日常的自发活动节律的过程<em>果蝇</em>和随后的数据分析。自发活动节律是一个可靠的动物生物钟的行为输出，时钟功能的标准读数研究昼夜的突变体或环境研究如何调节昼夜节律系统时使用。

Tags

Assaying Locomotor ActivityCircadian RhythmsSleep ParametersDrosophilaEndogenous Circadian PacemakersClock FunctionGenetic ScreensCellular And Biochemical BasesLocomotor MovementMonitoring SystemInfrared LightDaily Cycles Of Activity And InactivityCommercially Available Monitoring DevicesSoftware ProgramsData Analysis

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