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神経損傷を調べるための器官型海馬スライス培養モデル

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

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神経損傷を調べるための器官型海馬スライス培養モデル

18,891 Views

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06:33 min

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October 28, 2010

DOI:

DOI:

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06:33 min

October 28, 2010

•

5 Views

Qian Wang¹, Katrin Andreasson¹

¹Department of Neurology and Neurological Sciences,Stanford University School of Medicine

Summary

organoptypic海馬スライス培養モデルは、<em> in vitroで</em>モデルは、パラダイムの様々な神経損傷を調べるために使用。この記事では、スライス培養を生成し、神経損傷を定量化するための方法を説明します。

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Organotypic Hippocampal Slice Culture Neuronal Injury In Vitro Method Hippocampus Neuronal Architecture Composition Organotypic Culture System Astrocytic Effects Microglial Effects Ex Vivo Preparation Blood Flow Effects Peripheral Inflammatory Cells Excitotoxic Injury Hypoxic Injury Pyramidal Neurons Inflammatory Response Methods For Generating Hippocampal Slice Cultures Toxic Stimuli Assaying And Quantifying Neuronal Death

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