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采用芯片识别亚型特定的靶基因的全基因组分析
JoVE Journal
Biologia
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JoVE Journal Biologia
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
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采用芯片识别亚型特定的靶基因的全基因组分析

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11:19 min

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July 07, 2010

DOI:

10.3791/2101-v

DOI:
10.3791/2101-v

•

11:19 min
July 07, 2010

•

1 Views
, , , , , ,
1Department of Biochemistry and Molecular Genetics,University of Illinois Chicago – UIC, 2Research Unit on Biomedical Informatics,Universitat Pompeu Fabra, 3Genome Technology Core,Whitehead Institute for Biomedical Research

Summary

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在这里，我们提出的全基因组的不同在一个组蛋白结合结构域的蛋白异构体的位置分析染色质免疫沉淀（ChIP）的过程。我们把它应用到芯片的SEQ分析，以确定KDM5A/JARID1A/RBP2组蛋白去甲基化酶的目标。

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Cite this Article

Beshiri, M. L., Islam, A., DeWaal, D. C., Richter, W. F., Love, J., Lopez-Bigas, N., Benevolenskaya, E. V. Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets. J. Vis. Exp. (41), e2101, doi:10.3791/2101 (2010).

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