This content is Free Access.
PAR-Clip - En metod för att identifiera transkriptom hela bindningar på RNA-proteiner
52,829 Views
•
12:24 min
•
July 02, 2010
DOI:
1Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology,Rockefeller University, 2Berlin Institute for Medical Systems Biology,Max-Delbrück-Center for Molecular Medicine, 3Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 4Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 5Genomics Resource Center,Rockefeller University
Tags
PAR-CliPTranscriptome-wideBinding SitesRNA-binding ProteinsPost-transcriptional Gene RegulationRNA TranscriptsRNA-RBP InteractionsMicroRNA-containing Ribonucleoprotein ComplexesCell-type DependentlyInterplayHigh Resolution MapsIn Vivo Protein-RNA InteractionsGenetic ApproachesBiochemical ApproachesComputational ApproachesMicroarray ProfilingRIP-ChipKinetically Stable InteractionsRBP Recognition Element (RRE)Direct RBP Target Site InformationUV CrosslinkingImmunoprecipitationCLIPUV 254 Nm RNA-protein Crosslinking
Read Article
Cite this Article
Hafner, M., Landthaler, M., Burger, L., Khorshid, M., Hausser, J., Berninger, P., Rothballer, A., Ascano, M., Jungkamp, A., Munschauer, M., Ulrich, A., Wardle, G. S., Dewell, S., Zavolan, M., Tuschl, T. PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins. J. Vis. Exp. (41), e2034, doi:10.3791/2034 (2010).
Copy