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对活细胞成像的SNAP - Tag融合蛋白的荧光标记的COS - 7
JoVE Journal
Biologia
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JoVE Journal Biologia
Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
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对活细胞成像的SNAP - Tag融合蛋白的荧光标记的COS - 7

DOI:
10.3791/1876-v

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07:38 min

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May 17, 2010

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1Division of Chemical Biology,New England Biolabs

Capitoli

  • 00:00Titolo
  • 00:54Introduction
  • 01:14Transient Transfection of COS-7 Cells with pSNAP-ADRB2 and pSNAP-H2B
  • 03:25SNAP-Cell TMR Star Labeling of Transfected COS-7 Cells
  • 05:52Representative Results
  • 07:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

SNAP标签和剪辑标签蛋白标签制度，包括荧光染料分子，使特定的共价结合，一个活细胞中的兴趣的蛋白质。一旦克隆和表达，标记蛋白可用于许多下游应用的各种基材，而无需再次进行克隆。

Tags

Fluorescent LabelingCOS-7SNAP-tagFusion ProteinsLive Cell ImagingProtein Labeling SystemsCovalent AttachmentFluorescent DyesProtein Of InterestImaging InstrumentationCloningExpressionDownstream ApplicationsSNAP-tag SubstrateHuman O6-alkylguanine-DNA-alkyltransferase (hAGT)DNA Repair ProteinBenzyl LinkerLabeling ReactionCLIP-tagOrthogonal Labeling

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