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Isolation of Mouse Peritoneal Cavity Cells
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation of Mouse Peritoneal Cavity Cells

Isolation of Mouse Peritoneal Cavity Cells

DOI:
10.3791/1488-v

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04:32 min

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January 28, 2010

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1BloodCenter of Wisconsin,Blood Research Institute

Capitoli

  • 00:00Titolo
  • 00:41Introduction
  • 01:10Isolation of Cells from the Peritoneal Cavity
  • 03:35Resulting Population of Peritoneal Cavity Cells
  • 03:58Conclusion

Summary

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Traduzione automatica

The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.

Tags

Peritoneal Cavity CellsIsolationImmune CellsMacrophagesB CellsT CellsNaïve MacrophagesPeritoneal Cavity-resident B Cell SubsetB1 CellsB1a CellsB1b CellsCD11bCD5AutoreactivityAutoimmunityIL-10 Producing Capacity

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