Rivista
/
Immunologia e infezioni
/
补消耗的特定细胞群的枯竭
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Depletion of Specific Cell Populations by Complement Depletion
Please note that all translations are automatically generated. Click here for the English version.

补消耗的特定细胞群的枯竭

DOI:
10.3791/1487-v

•

06:17 min

•

February 05, 2010

•

1BloodCenter of Wisconsin,Blood Research Institute

Capitoli

  • 00:00Titolo
  • 00:45Introduction
  • 01:18Preparation of Rabbit Complement and Antibody Selection
  • 02:22Complement Depletion
  • 05:06Results of the Complement Depletion
  • 05:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

为了有效地研究其净化往往需要的人群的免疫细胞的功能。补枯竭是一个快速和廉价的技术，高纯度的免疫细胞群的隔离。

Tags

DepletionSpecific Cell PopulationsComplement DepletionImmune Cell PurificationReal-time PCRMicroarray AnalysisFluorescent Activated Cell Sorting (FACS)Magnetic Bead SeparationComplement SystemPlasma ProteinsProteolytic CascadeMembrane-attack ComplexCell DeathClassical PathwayIgM AntibodiesIgG AntibodiesMonoclonal Antibodies (mAb)Antigen-specific MannerRabbit ComplementIncubationWashing

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

特定细胞群的净化荧光激活细胞分选（FACS）

测量50％的溶血性补充（CH 50）活性的血清

富达的RNA病毒和病毒的突变频率的表征的变种的分离

在小鼠体内的巨噬细胞的枯竭和重建

免疫突触由双色时间选通受激发射损耗（STED）纳米显微可视化

定量检测抗体诱导补体激活的方法对红血细胞

杀手人工抗原提呈细胞（KaAPC）为有效<em>在体外</em>人抗原特异性T细胞耗竭

巨噬细胞胆固醇消耗及其对的吞噬作用<em>新型隐球菌</em

发电<em>德诺</em>抗原特异性人T细胞受体通过中心的Hemichain的逆转录病毒转导

利用受刺激的排放损耗 (STED) 显微镜可视化肌动蛋白和微管骨架在 B 细胞免疫突触中的应用

Read Article