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Non-רדיואקטיבי באתרו הכלאה פרוטוקול ישים עבור נורווגיה Spruce ומגוון של מיני צמחים
JoVE Journal
Biologia
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JoVE Journal Biologia
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
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Non-רדיואקטיבי באתרו הכלאה פרוטוקול ישים עבור נורווגיה Spruce ומגוון של מיני צמחים

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11:56 min

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April 17, 2009

DOI:

10.3791/1205-v

DOI:
10.3791/1205-v

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11:56 min
April 17, 2009

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1Department of Evolutionary Functional Genomics, Evolutionary Biology Center,Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter,Swedish University of Agricultural Sciences

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Non-radioactive In Situ HybridizationProtocolNorway SprucePlant SpeciesExpression Analysis TechnologiesExpression ProfilesIn Situ HybridizationMRNA ExpressionLaboriousTime-consumingOptimizationWoody SpeciesConiferPicea AbiesModified DIG In Situ Hybridization ProtocolRNase TreatmentProteinase K ConcentrationHomologous GenesMale Reproductive OrgansGymnospermAngiosperm SpeciesArabidopsis ThalianaBrassica NapusFloral OrgansMicrosporophylls

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Karlgren, A., Carlsson, J., Gyllenstrand, N., Lagercrantz, U., Sundström, J. F. Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species. J. Vis. Exp. (26), e1205, doi:10.3791/1205 (2009).

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