Rivista
/
Biologia
/
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
JoVE Journal
Biologia
Author Produced
This content is Free Access.
JoVE Journal Biologia
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

DOI:
10.3791/1205-v

•

11:56 min

•

April 17, 2009

•

, , , ,
1Department of Evolutionary Functional Genomics, Evolutionary Biology Center,Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter,Swedish University of Agricultural Sciences

Capitoli

  • 00:00Titolo
  • 00:45Fixation and Embedding
  • 01:41Sectioning
  • 06:16In Situ Hybridization
  • 11:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including Norway spruce. With just a few adjustments, including altered RNase treatment and proteinase K concentration, the protocol may be used in studies of different tissues and species.

Tags

Non-radioactive In Situ HybridizationProtocolNorway SprucePlant SpeciesExpression Analysis TechnologiesExpression ProfilesIn Situ HybridizationMRNA ExpressionLaboriousTime-consumingOptimizationWoody SpeciesConiferPicea AbiesModified DIG In Situ Hybridization ProtocolRNase TreatmentProteinase K ConcentrationHomologous GenesMale Reproductive OrgansGymnospermAngiosperm SpeciesArabidopsis ThalianaBrassica NapusFloral OrgansMicrosporophylls

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Double Whole Mount in situ Hybridization of Early Chick Embryos

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

RNA In situ Hybridization in Whole Mount Embryos and Cell Histology Adapted for Marine Elasmobranchs

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation

Read Article