JOVE JOURNAL

Bioingegneria

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Efficient Generation of Murine Chimeric Antigen Receptor (CAR)-T Cells

Rosa L. Vincent¹, Fangda Li², Edward R. Ballister¹, Nicholas Arpaia^2,3, Tal Danino^1,3,4

¹Department of Biomedical Engineering,Columbia University, ²Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons,Columbia University, ³Herbert Irving Comprehensive Cancer Center,Columbia University, ⁴Data Science Institute,Columbia University

Journal (Bioengineering)

High Throughput Screening Assessment of Reactive Oxygen Species (ROS) Generation using Dihydroethidium (DHE) Fluorescence Dye

Rahul Kumar^1,2, Rama R. Gullapalli^1,2

¹Department of Pathology,University of New Mexico, ²Department of Chemical and Biological Engineering,University of New Mexico

Journal (Bioengineering)

Dihydroethidium (DHE) Staining of Hepatocarcinoma Cells for the Quantification of Reactive Oxygen Species (ROS)

Rahul Kumar^1,2, Rama R. Gullapalli^1,2

¹Department of Pathology,University of New Mexico, ²Department of Chemical and Biological Engineering,University of New Mexico

Journal (Bioengineering)

3D Printing Bacteria to Study Motility and Growth in Complex 3D Porous Media

R. Kōnane Bay^1,2, Anna M. Hancock², Arabella S. Dill-Macky², Hao Nghi Luu², Sujit S. Datta²

¹Department of Chemical and Biological Engineering,University of Colorado Boulder, ²Department of Chemical and Biological Engineering,Princeton University

Journal (Bioengineering)

Bioprinting Bacterial Colonies for Motility and Growth Analysis in a Porous Hydrogel Matrix

R. Kōnane Bay^1,2, Anna M. Hancock², Arabella S. Dill-Macky², Hao Nghi Luu², Sujit S. Datta²

¹Department of Chemical and Biological Engineering,University of Colorado Boulder, ²Department of Chemical and Biological Engineering,Princeton University

Journal (Bioengineering)

A Practical Guide to Phage- and Robotics-Assisted Near-Continuous Evolution

Samir Aoudjane¹, Stefan Golas², Osaid Ather¹, Michael J. Hammerling³, Erika DeBenedictis¹

¹The Francis Crick Institute, ²Massachusetts Institute of Technology, ³Future House

Journal (Bioengineering)

Pre-Run Preparation for Phage- and Robotics-Assisted Near-Continuous Evolution (PRANCE) Technique

Samir Aoudjane¹, Stefan Golas², Osaid Ather¹, Michael J. Hammerling³, Erika DeBenedictis¹

¹The Francis Crick Institute, ²Massachusetts Institute of Technology, ³Future House

Journal (Bioengineering)

Hardware and Software Integration to Perform Phage- and Robotics-Assisted Near-Continuous Evolution (PRANCE)

Samir Aoudjane¹, Stefan Golas², Osaid Ather¹, Michael J. Hammerling³, Erika DeBenedictis¹

¹The Francis Crick Institute, ²Massachusetts Institute of Technology, ³Future House

Journal (Bioengineering)

Analyzing the Marker Trajectory Tracking and Strain Data of Peripheral Nerve Stretching

Virginia Orozco¹, Sriram Balasubramanian¹, Anita Singh²

¹School of Biomedical Engineering, Science and Health Systems,Drexel University, ²Bioengineering Department,Temple University

Journal (Bioengineering)

Direct Linear Transformation for the Measurement of In-Situ Peripheral Nerve Strain During Stretching

Virginia Orozco¹, Sriram Balasubramanian¹, Anita Singh²

¹School of Biomedical Engineering, Science and Health Systems,Drexel University, ²Bioengineering Department,Temple University

Journal (Bioengineering)

µSiM Device Assembly for Blood Brain Barrier Monoculture

Molly C. McCloskey¹, Pelin Kasap², Michelle Trempel¹, Louis P. Widom³, Julia Kuebel¹, Kaihua Chen¹, Thomas R. Gaborski³, Britta Engelhardt², James L. McGrath¹

¹Department of Biomedical Engineering,University of Rochester, ²Theodor Kocher Institute,University of Bern, ³Department of Biomedical Engineering,Rochester Institute of Technology

Journal (Bioengineering)

Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis

Molly C. McCloskey¹, Pelin Kasap², Michelle Trempel¹, Louis P. Widom³, Julia Kuebel¹, Kaihua Chen¹, Thomas R. Gaborski³, Britta Engelhardt², James L. McGrath¹

¹Department of Biomedical Engineering,University of Rochester, ²Theodor Kocher Institute,University of Bern, ³Department of Biomedical Engineering,Rochester Institute of Technology

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