Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.
Uniform and size-controllable poly (ethylene glycol) diacrylate (PEGDA) droplets could be produced via the flow focusing process in a microfluidic device. This paper proposes a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation. The polydimethylsiloxane (PDMS) chip was fabricated using the multi-layer soft lithography method. Hexadecane containing surfactant was used as the continuous phase, and PEGDA with the ultraviolet (UV) photo-initiator was the dispersed phase. Surfactants allowed the local surface tension to drop and formed a more cusped tip which promoted breaking into tiny micro-droplets. As the pressure of dispersed phase was constant, the size of droplets became smaller with increasing continuous phase pressure before dispersed phase flow was broken off. As a result, droplets with size variation from 1 µm to 80 µm in diameter could be selectively achieved by changing the pressure ratio in two inlet channels, and the average coefficient of variation was estimated to be below 7%. Furthermore, droplets could turn into micro-beads by UV exposure for photo-polymerization. Conjugating biomolecules on such micro-beads surface have many potential applications in the fields of biology and chemistry.
Droplet-based microfluidic systems have the ability to produce highly monodisperse droplets from nanometer to micrometer diameter range1 and hold great potential in the high-throughput drug discovery2, synthesis of biomolecules3,4, and the diagnostic testing5. Due to the unique advantages of smaller droplets, such as the greater surface area to volume ratio and the large-scale applications with consuming a few microliters of sample, the technology has attracted extensive interest in a broad range of fields. The emulsification of two immiscible liquids is one of the most typical methods to generate droplet. In previous reports in the field, researchers have developed a variety of different droplet formation geometries, including T-junction, flow-focusing and co-flowing geometries. In the T-junction geometry, the dispersed phase is delivered through a perpendicular channel into the main channel, in which the continuous phase flows6,7. In the typical two-dimensional (2D) flow-focusing8,9 geometry, the dispersed phase flow is sheared from the lateral; and for the co-flowing geometry10,11, on the other hand, a capillary introducing the dispersed phase flow is placed co-axially inside a bigger capillary for co-flowing geometry, so that the dispersed phase flow is sheared from all directions.
The droplet size is controlled by adjusting channel size and flow rate ratio, and the minimum size produced by co-flowing or T-junction is limited to dozens of micrometers. For flow-focusing droplet formation system, three modes of droplet breakup form by adjusting the pressure ratio of two-phase and surfactant concentration, including the dripping regime, the jetting regime, and tip-streaming15. Tip-streaming mode is also called thread formation, and the appearance of a thin the thread drawing out from the tip of dispersed phase flow cone will be observed.Previous studies have demonstrated droplets less than a few micrometers could be generated though tip-streaming process in 2D or semi-3D flow-focusing device8,12. However, as an aqueous solution containing a very low concentration of PEGDA was used as the dispersed phase, the shrinkage ratio of PEGDA particles was about 60% of the original droplets in diameter after photo-polymerization, while PEGDA without dilution as the dispersed phase led to unstable tip-streaming mode12. Interfacial tension is an important parameter of emulsion process and it will decrease due to the addition of the surfactant into the continuous phase liquid,leading to decrease in droplet size, higher generation frequency13, highly curved tip, and preventing instability14. Furthermore, when the bulk surfactant concentration is much higher than the critical micelle concentration, the interfacial tension is approximately invariable in the saturated state13 and the tip-streaming mode can occur15.
Based on the above observations, in this paper, we developed a facile approach for PEGDA droplets generation using a semi-3D flow-focusing microfluidic device, fabricated by multi-layer soft lithography method. Different from the typical 2D flow-focusing device, the semi-3D flow-focusing device has a shallow dispersed phase channel and a deep continuous phase channel, so that the dispersed phase can be sheared from up and down beside lateral. This provides larger adjusting range for flow-focusing mode by reducing the energy and pressure required for droplet breakup. Different from the previous report12, the dispersed phase is pure PEGDAcontaining photo-initiator, making sure that the shrinkage ratio of PEGDA particles is lower than 10%16; and the continuous phase is the mixture of hexadecane dissolving with a high bulk concentration of the silicone-based nonionic surfactant. Size-controllable and uniform droplets were produced by adjusting the pressure ratio of two phases. The diameter of the droplets changes from 80 µm to 1 µm as the droplet breakup processes changes from the jetting mode to tip-streaming mode. In addition, the PEGDA particle was synthesized through photo-polymerization process under UV exposure. The droplet generation microfluidic system with ease of fabrication will provide more possibilities for biological applications.
1. Mold Fabrication
2. Semi-3D Flow-focusing Microfluidic Chip Fabrication
3. Reagents Preparation
4. System Preparation
5. Droplets Formation
6. PEGDA Particles Collection and Characterization
The semi-3D flow-focusing microfluidic chip was fabricated using multi-layer soft lithography techniques as described above. The fabrication process and results for master mold in the protocolare shown in Figure 2. The first layer, which provides a 65 µm wide channel for introducing the dispersed phase and a 50 µm wide orifice (Figure 2a), is 20 µm in thickness. An addition 130 µm thickness layer is used to provide the continuous phase channel and the exit channel (Figure 2b). Figure 2c shows a finished mold. A filter in the inlet is designed to prevent debris of the holes punched in the PDMS from entering. This is done to overcome clogging in the orifice (Figure 2d).
After fabricating the master molds, the casting process and results in the protocol are shown in Figure 3. Top and bottom half-pieces with mirrored structures are prepared using PDMS. Using a 0.75 mm punch to drill the inlet and outlet holes into the top layer of PDMS. After oxygen plasma treatment simultaneously, the features of the top and the bottom PDMS slabs are aligned with less alignment error which does not significantly affect the device performance. The length of the whole semi-3D device is about 5 cm. We tried the 10 cm long chips adding the downstream channel. However, the larger the chip, the more difficult the alignment process due to increasing alignment region. In addition, the shorter chip (such as 2.5 cm long chip we used) also makes alignment process difficult due to lack of flexibility.
The semi-3D flow-focusing microfluidic device and the typical droplet formation process are illustrated in Figure 4. Because of the difference of depth of the dispersed phase channel and the continuous phase channel, the dispersed phase flow is expected to be squeezed from all the directions by the continuous phase flow. As a result, symmetric conical liquid tip forms to produce droplets continuously. The size of droplets is changed by the pressure ratio of dispersed and continuous phases flow. For our experiments, the pressure of dispersed phase (PD) is maintained constant as the base-level pressure, and the pressure of continuous phase (PC) is modified to affect the shearing force, so that the droplet breakup processes change from the jetting mode to the tip-streaming mode, as shown in Figure 5. The droplets are solidified by photo-polymerization forming particles. UV exposure polymerizes the monomer in the droplets. Figure 6 shows fluorescent images of particles with different pressure ratio; and image analysis reveals the droplet size, which is plotted as a function of the pressure ratio in Figure 7a. By analogy with the electric circuit method24, the equivalent fluidic circuit is shown in the following figure 7b. We roughly calculated the hydraulic resistance of three parts: the dispersed phase channel is 1.26 X 1014Pa • s • m-3 (R3); the sum of the orifice and downstream channel is 6.08 X 1012Pa • s • m-3 (R4+ R5); the continuous phase channel and the filter are 2.19 X 1012Pa • s • m-3 (R2)and 1.10 X 1012Pa • s • m-3 (R1). The relationships between all hydraulic resistances and flow rates are shown as following:
PB is the pressure of intersection of two phases microchannel. When the pressure of dispersed phase (PD) is maintained at 45 mbar, the pressure ratio is converted to corresponding flow rate ratio:
QC = 0.8859PC – 1.62891
QD = 2.1302 – 0.0217PC
The droplet size is plotted as a function of the flow rate ratio in figure 7c. The figure indicates that the increasing pressure ratio (PC / PD) leads to the dispersing phase flow being sheared into the spindlier tip and the decreasing droplet. The size range of the PEGDA particles varies from 1 µm to 80 µm with an average coefficient of variation (CV) below 7%. The smaller droplets were observed through the fluorescent microscope with 60X object, so there were only a dozen or so droplets in the view of the microscope. In addition, smaller droplets were approximately twenty or thirty pixels in radius. It was hard to characterize the coefficients of variance of smaller droplets, and the small base would lead to an inaccurate calculation, so the CVs of those smaller droplets were not indicated.
Figure 1: The configuration of experimental system Please click here to view a larger version of this figure.
Figure 2: Mater molds for multi-layer soft lithography. (a) The mask 1 used for formation of 20 µm features. The master contains the dispersed phase channel and an orifice. (b) the mask 2 used for formation of 130 µm features. The master contains the continuous phase channel and the exit channel. (c) Monolithic master. (d) CAD drawing and SEM of the filter, located at the inlet to prevent clogging. Please click here to view a larger version of this figure.
Figure 3: Casting and bonding processes for the PDMS microfluidic chip. (a) Schematic diagram of the assembly of the semi-3D PDMS device. (b) Structures of PDMS slab under SEM.(c) Monolithic microfluidic device. Please click here to view a larger version of this figure.
Figure 4: An Illustrative working principle of the semi-3D flow-focusing microfluidic systems. Please click here to view a larger version of this figure.
Figure 5: An Illustrative fluorescence mages of various droplet breakup processes. (a-d) the jetting mode and (e-f) the tip-streaming mode. PC is the pressure of continuous phase, and PD is the pressure of dispersed phase. Please click here to view a larger version of this figure.
Figure 6. PEGDA particles under different pressure ratio. Fluorescence images of particles in different sizes and particles under (a-b) the optical microscope and (c-d) confocal laser scanning microscopy. PC is the pressure of continuous phase, and PD is the pressure of dispersed phase. Please click here to view a larger version of this figure.
Figure 7. Droplet size. (a) Corresponding sizes on the basis of the pressure ratio. Black square represents the droplet size distribution, and the superscript numbers are the corresponding coefficient of variation. Smaller droplets are hard to characterize the coefficients of variance of smaller droplets, so the CV of those smaller droplets were not indicated. (b) Illustration of fluidic circuit. (c) The relationship between droplet size and flow rate ratio. Please click here to view a larger version of this figure.
The generation of droplets in the flow-focusing mode using 2D and semi-3D microfluidic device has previously been developed in a variety of reports8,9,15,19,20,21. In these systems, the aqueous liquid that could not be solidified was chosen as the dispersed phase, such as deionized water8,15,20,21, an aqueous solution of sodium hydroxide19 and the formation of stable tip-streaming mode needs the support of high voltage electric field8,21. In addition, such flow-focusing droplet formation system is similar to the one of pure water, with a low concentration aqueous solution of PEGDA12, which is more stable than the one of using PEGDA without dilution as the dispersed phase.
In our semi-3D flow-focusing microfluidic system without high voltage electric field, undiluted PEGDA solution was used as the dispersed phase liquid, increasing the difficulty to form stable droplet breakup process. We found that the tip-streaming mode was more stable by increasing the concentration of surfactant; and also, increasing the concentration of surfactant decreased the local surface tension and formed a more cusped tip, leading to the droplet size decrease. As a result, size-controllable (1 µm to 80 µm in diameter) droplets can be obtained by only adjusting the pressure ratio, in an ease of fabrication and high reproducibility manner.
However, there is a major restriction for our semi-3D flow-focusing microfluidic system. PDMS is a kind of flexible material so that the flow-focusing mode would become unstable under high pressure due to deformation of the microchannel. In addition, although it was reported that hexadecane would cause PDMS swelling22, we didn’t observe significant deformation of our microchannel causing by such effect.The 80 µm and 100 µm wide channel for the dispersed phase were selected, and slight deformation was observed when the pressure increased. So, we suggest that the flow rate in the orifice region is too high under such high pressure, leading to the inevitable deformation, but not due to the swelling effect of hexadecane. A whole flat device will bend slightly after continued use for 7 hours. It takes about 4 hours to measure a group of practical data, and the device has not been deformed remarkably. Furthermore, it is worth exploring whether the fiercer breakup process using T-junction resulted in the unstable flow-focusing mode. Y-junction, the flow-focusing structure with an angle between two phase channels (including 15°, 45°, 65°) was selected to make a gentle flow-focusing for a more stable mode. However, no tip-streaming mode occurred under those microfluidic devices, and only bigger droplets formed under jetting mode. It also was reported that the full width of dispersed phase flow was about 30 µm under high-pressure ratio using Y-junction23. Finally, the base-level pressure applied on the dispersed phase was somewhat low, and low pressure reduces the generation frequency, especially for the smaller droplets. Higher production rate is expected to be acquired through the paralleled structure in our future work.
Smaller droplet causes higher surface-volume ratio, leading to higher reaction rate and efficiency. In biology, the small droplet will be used for antibody screening and drug discovery by surficial decoration, encapsulation by adding biological molecules, such as targeted genetic and cells, and producing functional particle by adding magnetic and fluorescent material. We hope that our protocols, relating to fabrication of semi-3D flow-focusing PDMS device and small droplet generation, will contribute to continuous and deeper studies in such field, and be used in wide range of biological applications.
The authors have nothing to disclose.
This work was supported by the Shenzhen fundamental research funding (Grant No. JCYJ 20150630170146829, JCYJ20160531195439665 and JCYJ20160317152359560). The authors would like to thank Prof. Y. Chen at the Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences for supports.
Silicon wafer | Huashi Co., Ltd | ||
SU-8 2025, 2100 | Microchem Co. | Y111069 | |
SU-8 developer | Microchem Co. | Y020100 | |
Chromium mask | Qingyi Precision Mask Making Co., Ltd | ||
polydimethylsiloxane(PDMS) | Dow Corning | Sylgard 184 | |
poly(ethylene glycol) diacrylate (PEGDA) | Sigma | 26570-48-9 | |
2-hydroxy-40-(2-hydroxyethoxy)-2-methylpropiophenone | TCI | H1361-5G | photoinitiator |
Hexadecane | Sigma | 544-76- 3 | |
ABIL EM 90 | CHT | 144243-53-8 | surfactants |
Rhodamine B | Aladdin | 81-88-9 | fluorescent dye |
Spin Coater | |||
Lithography machine | |||
Automatic ointment agitator | Thinky | ARV-310 | |
Oven | BluePard | ||
Optical microscope | OLYMPUS | IX71 | |
High-speed camera | Hamamatsu, Japan | ORCA-flash | |
MAESFLO Microfluidic Fluid Control System | FLUIGENT | MFCS-EZ | |
UV lamp | FUTANSI | 365 nm UV light, 8000 MW/CM2 |