Generating Plasma Membrane Vesicles from Bone Marrow Stem Cells
Published: May 31, 2024
Abstract
Source: Xu, L. et al., Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy. J. Vis. Exp. (2017)
This video demonstrates a method to generate plasma membrane vesicles from bone marrow stem cells by passing them rapidly through a membrane filter. These PMVs show potential for cytoplasm replacement therapy.
Protocol
1. Assembly of the Apparatus To ensure sterility, turn on the ultraviolet (UV) light of a tissue culture hood for 30 min before use. Unscrew a disposable 25 mm filter unit and submerge the cap and bottom of the unit into a 200 mL glass cup filled with 75% ethanol for 30 min. The unit is made of medical-grade polypropylene. Pick up the cap and bottom of the unit using forceps, shake off the remaining ethanol by hand, and allow the parts to air dry for 10 min in the hood.</l…
Divulgazioni
The authors have nothing to disclose.
Materials
| IsoporeTM membranes | Millipore | TSTP04700 | 3 mm pore |
| Disposable filter unit | Xinya, Shanghai, China | 25 mm | Medical grade polypropylene |
| Insulin syringe | BD | 328446 | 1 ml |
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Citazione di questo articolo
Generating Plasma Membrane Vesicles from Bone Marrow Stem Cells. J. Vis. Exp. (Pending Publication), e22270, doi: (2024).