A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation
Published: November 30, 2023
Abstract
Source: Coats, J. S. et al., Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line. J. Vis. Exp. (2017)
In this video, we demonstrate a tail-snipping procedure in a mouse model to collect peripheral blood. The collected blood is subsequently subjected to centrifugation to separate plasma, which can be used for further experiments.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Serial Blood Collection and Plasma Monitoring for Exogenous Human Cytokine in Mice Blood collection via tail snip Disinfect the biosafety hood and assemble the mouse restrainer, scissors, and other tools/supplies for the procedure. Place the mouse in a re…
Divulgazioni
The authors have nothing to disclose.
Materials
| Animal Implantable Nano Transponder with Canula | Trovan | ID-100B(1.25) | |
| EMLA lidocaine anesthetic cream (obtain by presciption through animal care facility) | perscription | perscription | |
| BD ½ cc LO-DOSE U-100 Insulin Syringe 28G½ | BD | #329461 | |
| BD Microtainer Tubes with K2EDTA | BD | #365974 | |
| Centrifuge | Beckman Coulter | Alegra X-15R | |
| FisherBrand Capillary Tubes (Heparinized) | Fisher Scientific | 22-260-950 | |
| Hemocytometer | Fisher | 02-671-6 | |
| MACSQuant Analyzer 10 | Miltenyi Biotec | 130-096-343 | |
| Mouse Pie Cage | Braintree Scientific, Inc. | MPC-1 | |
| Mouse Tail Illuminator Restrainer | Braintree Scientific, Inc. | MTI STD | |
| Pistol Grip Implanter | Trovan | IM-300(1.25) | |
| "NSG mice" NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ, male or female, age 6 – 8 weeks | JAX Mice | # 005557 |
Citazione di questo articolo
A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation. J. Vis. Exp. (Pending Publication), e21836, doi: (2023).