Oral Intubation of Adult Zebrafish to Administer Experimental Compounds
Published: October 31, 2023
Abstract
Source: Ji, J., Thwaite, R. et al. Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds. J. Vis. Exp. (2018).
This video demonstrates a technique for orally intubating adult zebrafish to administer experimental compounds into the esophagus and monitor their intestinal uptake. This method offers a valuable model for studying the dynamics of oral prophylactics or other compounds within the intestine.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board 1. Solutions Required Prepare 150 mg/L (for anesthesia) or 300 mg/L (for euthanasia) of ethyl 3-aminobenzoate methanesulfonate (MS-222) solution with water from the aquarium where the zebrafish are maintained. Fill a small tank with 1 L of anesthetic solution and keep it aerated. Fill another small tank with 1 L o…
Divulgazioni
The authors have nothing to disclose.
Materials
| Silicone tube | Dow Corning | 508-001 | 0.30 mm inner diameter and 0.64 mm outer diameter |
| Luer lock needle | Hamilton | 7750-22 | 31 G, Kel-F Hub |
| Luer lock syringe | Hamilton | 81020/01 | 100 μL, Kel-F Hub |
| Filtered pipette tip | Nerbe Plus | 07-613-8300 | 10 μL |
| MS-222 | Sigma Aldrich | E10521 | powder |
| 10x PBS | Sigma Aldrich | P5493 | |
| Filter paper | Filter-Lab | RM14034252 | |
| Atto-488 NHS ester | Sigma-Aldrich | 41698 | |
| DMEM | Gibco | 31966 | Dulbecco's modified eagle medium |
| Maxwell RSC simplyRNA Tissue Kit | Promega | AS1340 |
Tags
Citazione di questo articolo
Oral Intubation of Adult Zebrafish to Administer Experimental Compounds. J. Vis. Exp. (Pending Publication), e21757, doi: (2023).