Intracisternal Delivery of Meningococci into Mouse Models to Induce Meningococcal Meningitis
Published: August 31, 2023
Abstract
Source: Pagliuca, C., et al., Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery. J. Vis. Exp. (2019)
In this video, we demonstrate the intracisternal delivery of Neisseria meningitidis into a mouse model to generate meningitis infection.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Intra-cisternal injection of mice NOTE: The entire procedure is performed in the laminar flow cabinet to maintain aseptic conditions. House laboratory mice (8-week-old, female Balb/c) under specific pathogen-free conditions. Provide food pellets and water ad libitum….
Divulgazioni
The authors have nothing to disclose.
Materials
| Alarm-Thermometer | TESTO | 9000530 | Device |
| BD Micro Fine syringe | BD | 320837 | U-100 Insulin |
| BD Plastipak syringe 1ml 25GA 5/8in | BD | 300014 | 05x16mm |
| BD Plastipak syringe 5ml | BD | 308062 | 07 x 30mm |
| BIOHAZARD AURA B VERTICAL LAMINAR FLOW CABINET | Bio Air s.c.r.l. | Aura B3 | Device |
| C150 CO2 Incubator | Binder | 9040-0078 | Device |
| Diete Standard Certificate | Mucedola s.r.l. | 4RF21 | Food pellet for animal |
| Dumont Hp Tweezers 5 Stainless Steel | F.S.T. by DUMONT | AGT5034 | 0,10 x 0,06 mm tip |
| Electronic Balance | Gibertini | EU-C1200 | Max 1200g, d=0.01g, T=-1200g |
| Iron dextran solution | Sigma-Aldrich | D8517-25ML | |
| Ketamine | Intervet | ||
| Microbiological Safety Cabinet BH-EN and BHG Class II | Faster | BH-EN 2004 | |
| Microcentrifuge tubes 1.5ml | BRAND | PP780751 | Screw cap PP, grad |
| Mouse Handling Forceps | F.S.T. | 11035-20 | Serrated rubber; Gripping surface:15 x 20 mm |
| Natural Latex Gloves | Medica | M101 | |
| Touch N Tuff disposible nitrile gloves | Ansell | 92-500 | |
| Xylazine | Intervet |
Tags
Citazione di questo articolo
Intracisternal Delivery of Meningococci into Mouse Models to Induce Meningococcal Meningitis. J. Vis. Exp. (Pending Publication), e21602, doi: (2023).