Superior Lacrimal Gland Removal: A Surgical Technique to Remove the Palpebral and Orbital Parts of Superior Lacrimal Gland from Rabbit Model
Published: April 30, 2023
Abstract
Source: Honkanen, R. A. et al., Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits. J. Vis. Exp. (2020).
This video describes the surgical procedure to remove the two parts: palpebral and orbital of the superior lacrimal gland from a rabbit to create a dry eye disease model. This rabbit model is suitable for studies of ocular surface homeostasis, pathophysiology and ocular therapeutics.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. Use New Zealand White (NZW) rabbits weighing 2−3 kg Lightly sedate animals placed in a restraining bag with subcutaneous acepromazine as above (1 mg/kg). Remove all fur on the face and dorsal surface of the skull to visualize the surgical landmarks. Trim fur with cutting shears leaving residual fine fur about 1 mm in length. Remove all residual …
Representative Results
Figure 1: Removal of the OSLG. (A) Surgical sites are infiltrated with anesthetic using a 50:50 combination of 2% lidocaine with 1:100,000 epinephrine and 0.5% bupivacaine, which is injected into the upper lid and along the incision lines to minimize discomfort during the procedure. (B) A Colorado microdissection needle is used to incise the skin and superfi…
Divulgazioni
The authors have nothing to disclose.
Materials
| acepromazine, Aceproinj | Henry Schein Animal Health, Dublin, OH | NDC11695-0079-8 | 0.1ml/kg subcutaneously injection for rabbit sedation |
| animal restraining bag | Henry Schein Animal Health, Dublin, OH | Jorvet J0170 | Use appropriately sized bag. |
| bupivacaine, 0.5% | Hospira Inc, Lake Forest IL | NDC: 0409-1162-02 | Mixed 50:50 with 2% lidocaine with 1:100,000 epinephrine for infiltration of incision sites. |
| Colorado needle | Stryker Craniomaxillofacial, Kalamazoo, MI | N103A | Use with electrosurgical unit to make incisions |
| electrosurgical unit with monopolar cautery plate | Valleylab, Boulder, CO | Force FXc | Use with electrosurgical unit to make incisions |
| forceps, Bishop Harmon | Bausch and Lomb (Storz), Bridgewater | NJ E1500-C | Use toothed forceps for dacryoadenectomy |
| needle, 30-gauge | BD, Franklin Lakes, NJ | REF 305106 | For infiltration of incision sites; syringe and needle size are not critical |
| rabbit, New Zealand White | Charles River Labs, Waltham, MA | (NZW) 2-3 kg | Research animals |
| scissors, Vannas McKesson | Medical-Surgical, San Francisco, CA | Miltex 2-130 | Capsulotomy scissors for dacryoadenectomy |
| syringe, 5 cc | BD, Franklin Lakes, NJ | REF 309603 | For infiltration of incision sites; syringe and needle size are not critical |
Tags
Citazione di questo articolo
Superior Lacrimal Gland Removal: A Surgical Technique to Remove the Palpebral and Orbital Parts of Superior Lacrimal Gland from Rabbit Model. J. Vis. Exp. (Pending Publication), e20846, doi: (2023).