Nictitating Membrane Removal: A Surgical Procedure to Remove Nictitating Membrane from Rabbit Eye
Published: April 30, 2023
Abstract
Source: Honkanen, R. A., et al. Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits. J. Vis. Exp. (2020).
In this video, we demonstrate a surgical procedure to remove nictitating membrane from rabbit’s eye.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Removal of the nictitating membrane NOTE: For simplicity, the technique for the right eye is described below. Complete this procedure on the left eye in an identical manner. Remove the nictitating membrane bilaterally during the acclimation period (usually the first week). Place rabbit in an …
Divulgazioni
The authors have nothing to disclose.
Materials
| acepromazine, Aceproinj | Henry Schein Animal Health, Dublin, OH | NDC11695-0079-8 | 0.1ml/kg subcutaneously injection for rabbit sedation |
| animal restraining bag | Henry Schein Animal Health, Dublin, OH | Jorvet J0170 | Use appropriately sized bag. |
| cautery unit, high-temperature, battery-powered | Medline Industries Inc, Northfield, IL | REF ESCT001 | Keep on hand in case of bleeding, protocol 2.7 |
| forceps, 0.3 | Bausch and Lomb (Storz), Bridgewater, NJ | ET6319 | For removal of nictating membrane, protocol 2.5 |
| lid speculum, wire | Bausch and Lomb (Storz), Bridgewater, NJ | Barraquer SUH01 | For removal of nictating membrane, protocol 2.4 |
| lidocaine 2% with epinephrine 1:100,000; 50:50 mixture | Hospira Inc, Lake Forest IL | NDC 0409-3182-02 | Pre-treat before removal of nictating membranes, protocol 2.4 |
| lidocaine, preservative-free | Sigma-Aldrich, St. Louis, MO | L5647 | 1% in PBS for anesthesia agent, for application to eye, protocol 2.4 |
| micropipette | Eppendorf | Research Plus 100 uL | For application of preservative-free lidocaine to eye, protocol 2.4 |
| micropipette tips | World Wide Medical Products | 41071052 | For application of preservative-free lidocaine to eye, protocol 2.4 |
| needle, 26-gauge | BD, Franklin Lakes, NJ | REF 305115 | For injection of lidocaine/epinephrine, protocol 2.3 & 2.5 |
| rabbit, New Zealand White | Charles River Labs, Waltham, MA (NZW) | 2-3 kg | Research animals |
| scissors, Westcott tenotomy | McKesson Medical-Surgical, San Francisco, CA | Miltex 18-1480 | For removal of nictating membrane, protocol 2.7 |
| syringe, 1 cc | BD, Franklin Lakes, NJ | ref 309659 | For injection of lidocaine/epinephrine, protocol 2.3 & 2.5 |
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Citazione di questo articolo
Nictitating Membrane Removal: A Surgical Procedure to Remove Nictitating Membrane from Rabbit Eye. J. Vis. Exp. (Pending Publication), e20845, doi: (2023).