Apical Resection Neonatal Mouse Model: A Surgical Procedure to Amputate the Ventricular Apex to Study Regenerative Potential in a Neonate Mouse Heart

Published: April 30, 2023

Abstract

Source: Li, Y. et al. Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice. J. Vis. Exp. (2021).

In this video, we demonstrate the procedure to surgically remove the left ventricular apex of a neonatal mouse heart.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Apical resection operation in neonatal 1-day-old Cx3cr1 GFP/+ mouse (C57BL/6 background) Anesthesia Take all the mouse pups out from the cages and put them in a clean dry box. Embed each mouse in the ice for about 2-3 minut…

Divulgazioni

The authors have nothing to disclose.

Materials

Neonatal Heart Dissociation Kit  Miltenyi Bio Tech, Teterow, Germany  130-098-373

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Citazione di questo articolo
Apical Resection Neonatal Mouse Model: A Surgical Procedure to Amputate the Ventricular Apex to Study Regenerative Potential in a Neonate Mouse Heart. J. Vis. Exp. (Pending Publication), e20803, doi: (2023).

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