Intact Ovary Harvest from Mouse: A Surgical Procedure to Isolate Complete Ovary from Murine Model

Published: April 30, 2023

Abstract

Source: Mason, J. B. et al. Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice. J. Vis. Exp. (2018)

In this video, we describe a surgical procedure to harvest intact ovary from a mouse model.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Transplantation of intact ovaries to aged mice NOTE: This protocol describes the procedure used during the transfer of ovaries from a young mouse (60 days of age) to a post reproductive female (12 months of age) of the same inbred strain. The same procedure is used for ovarian transplantat…

Divulgazioni

The authors have nothing to disclose.

Materials

Dissecting microscope Any dissecting scope with an adjustable working distance.
Warm Water Recirculator and appropriate pad for your size surgical/recovery field. Stryker/Gaymar TP700 Circulating water pads produce even and consistent temperature operating and recovery fields.
Surgical instruments-dissection instruments for access to the reproductive tract Choice of specific surgical instruments is highly surgeonspecific
Suture material for ovarian bursa eSutures 2809G: 9-0 or 2810G: 10-0 ETHILON BLACK 5" BV130-5 TAPER 10-0 suture can be difficult to locate if dropped on the surgery field.
Absorbable gelatin sponge PFIZER Gelfoam- PFIZER 09-0315-08 cut into small (~2mm) pieces under sterile conditions.
Staples for skin closure Becton Dickinson MikRon Autoclip 9mm (BD 427631)
Eye ointment. MWI Veterinary Supply Co. Puralube sterile opthalmic ointment
Syringes for anesthesia, analgesia and epinephrine MWI Veterinary Supply Co BD 305536 27g x 3/8in,Intradermal Bevel 1/2ml
Syringes for saline. MWI Veterinary Supply Co BD 309657. 3ml syringe, luer-lok tip, 27g needle
Clippers/blade Andis Super AGR+™ Cordless Detachable Blade Clipper and a CeramicEdge® #40 blade. Any clippers with a fine blade.
Ethanol University chemical supply dilute to 70% for use.
Betadine solution MWI Veterinary Supply Co. 3233
2×2 gauze pads MWI Veterinary Supply Co. 3073 autoclaved with surgery packs
Borosilicate glass beads Sigma-Aldrich Co Z273619 Autoclave in sintilation vials
4-vinylcyclohexene diepoxide Sigma-Aldrich Co. 94956

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Citazione di questo articolo
Intact Ovary Harvest from Mouse: A Surgical Procedure to Isolate Complete Ovary from Murine Model. J. Vis. Exp. (Pending Publication), e20793, doi: (2023).

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