Zebrafish Cardiomyopathy Model: Drug-Induced Cardiotoxicity
Published: April 30, 2023
Abstract
Source: Ma X., et. al. A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish. J. Vis. Exp. (2018).
This video describes the technique of creating a zebrafish cardiomyopathy model with minimal variations among experimental groups and in the sample, we create zebrafish cardiomyopathy model using the drug doxorubicin.
Protocol
1. DOX Injection in Adult Fish Place a clean 100 mm Petri dish with a sponge inside it, under a dissection microscope, then adjust the focus. Cut the sponge to make a cavity of about 4 cm in length to hold a fish. Make a longer cavity for a larger fish. Prepare a 34 G needle with a 10 µL micro-syringe. Rinse the needle with 1x HBSS buffer to remove any bubbles and blocks from the syringe and tubing. Anesthetize the adult fish in embryo water containing 0.16 mg/mL tricaine for 2 min.<b…
Materials
| Crossing tank | Aquaneering | ZHCT100 | Fish breeding |
| Doxorubicin hydrochloride | Sigma | D1515-10MG | |
| Hank's balanced salt solution (HBBS) | ThermoFisher | 14025076 | Vehicle for DOX |
| 100 mm petri dish | Falcon | 431741 | |
| 10 μL NanoFil micro-syringe | WPI | NANOFIL | For injection |
| 34 gauge needle | WPI | NF34BV-2 | For injection |
| Tricaine | Argent | Anesthetizing fish |
Citazione di questo articolo
Zebrafish Cardiomyopathy Model: Drug-Induced Cardiotoxicity. J. Vis. Exp. (Pending Publication), e20202, doi: (2023).