Chapter 32
Analyzing Cells and Proteins
![Overview Of Cell Separation And Isolation](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13371.jpg)
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years…
![Cell Culture](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13372.jpg)
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are…
![Hybridoma Technology](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13374.jpg)
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic…
![Tissue Homogenization and Cell Lysis](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13830.jpg)
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular…
![Flow Cytometry](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13379.jpg)
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a…
![Principles Of Column Chromatography](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13376.jpg)
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents.…
![Immunoprecipitation](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13377.jpg)
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their…
![Tagging and Fusion Proteins](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13378.jpg)
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated…
![Western Blotting](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13381.jpg)
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting…
![Two-dimensional Gel Electrophoresis](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13382.jpg)
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This…
![MALDI-TOF Mass Spectrometry](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13384.jpg)
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to…
![Peptide Identification Using Tandem Mass Spectrometry](https://jovecdn2.ccindex.cn/files/media/science-education/science-education-thumbs/13385.jpg)
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass…
![X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease](https://jovecdn2.ccindex.cn/files/thumbs/59559_t.png)
X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease
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Transgenic mouse models have been important tools for studying the relationship of genotype to phenotype for human diseases including those of…
![Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization](https://jovecdn2.ccindex.cn/files/thumbs/60393_t.png)
Interferon regulatory factor 5 (IRF5) is a key transcription factor for regulating the immune response. It is activated downstream of the Toll-like…