JOVE JOURNAL

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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Mithun Mitra^1,2, Ha Neul Lee^1,2,3, Hilary A. Coller^1,2,3

¹Department of Molecular, Cell and Developmental Biology,University of California, Los Angeles, ²Department of Biological Chemistry,David Geffen School of Medicine, ³Molecular Biology Institute Interdepartmental Program,University of California, Los Angeles

Journal (Genetics)

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Meagan Jezek¹, Alison Jacques¹, Deepika Jaiswal¹, Erin M. Green¹

¹Department of Biological Sciences,University of Maryland

Journal (Genetics)

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Ming Li^1,2, Michelle Bui^1,2, Omar S. Akbari^1,2

¹Department of Entomology and Riverside Center of Disease Vector Research, Institute for Integrative Genome Biology,University of California, Riverside, ²Section of Cell and Developmental Biology, Division of Biological Sciences,University of California, San Diego

Journal (Genetics)

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

Sriram Vijayraghavan¹, Boris Kantor¹

¹Duke Viral Vector Core, Department of Neurobiology,Duke University School of Medicine

Journal (Genetics)

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Alireza Lorzadeh¹, Rodrigo Lopez Gutierrez¹, Linda Jackson¹, Michelle Moksa¹, Martin Hirst^1,2

¹Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology,University of British Columbia, ²Canada's Michael Smith Genome Science Center,BC Cancer Agency

Journal (Genetics)

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Anna Köferle¹, Stefan H. Stricker^1,2

¹MCN Junior Research Group, Munich Center for Neurosciences,Ludwig-Maximilian-Universität, BioMedical Center, ²Epigenetic Engineering, Institute of Stem Cell Research,Helmholtz Zentrum, German Research Center for Environmental Health

Journal (Genetics)

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Ivana Grbesa¹, Miriam Tannenbaum¹, Avital Sarusi-Portuguez¹, Michal Schwartz¹, Ofir Hakim¹

¹The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Journal (Genetics)

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Yuhui Chen¹, Xianfu Wang², Shunfei Lu³, Hongcheng Wang², Shibo Li², Rujin Chen¹

¹Laboratory of Plant Genetics and Development,Noble Research Institute, ²Genetics Laboratory,University of Oklahoma Health Science Center, ³Medicine and Health School,Li Shui University

Journal (Genetics)

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Timothy L. Hostelley*¹, Jessica E. Nesmith*¹, Norann A. Zaghloul¹

¹Division of Endocrinology, Diabetes and Nutrition, Department of Medicine,University of Maryland School of Medicine

Journal (Genetics)

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Takatoshi Murata¹, Ayako Okada¹, Khairul Matin^1,2,3, Nobuhiro Hanada¹

¹Department of Translational Research,Tsurumi University School of Dental Medicine, ²Endowed Department of International Oral Health Science (affiliated with Department of Translational Research),Tsurumi University School of Dental Medicine, ³Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences,Tokyo Medical and Dental University

Journal (Genetics)

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

Allison Jandura^1,2, Jack Hu¹, Ronit Wilk^1,2, Henry M. Krause^1,2

¹The Terrence Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, ²Department of Molecular Genetics,University of Toronto

Journal (Genetics)

The Nematode Caenorhabditis Elegans – A Versatile In Vivo Model to Study Host-microbe Interactions

Luca Issi¹, Meredith Rioux¹, Reeta Rao¹

¹Biology and Biotechnology,Worcester Polytechnic Institute

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