JOVE JOURNAL

Biochimica

Showing 409 - 420 in 778 videos.

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Ying Fan*¹, Francesca Tonelli*¹, Shalini Padmanabhan², Marco A.S. Baptista², Lindsey Riley², Danielle Smith³, Connie Marras⁴, Andrew Howden⁵, Dario R. Alessi¹, Esther Sammler^1,6

¹MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, ²The Michael J. Fox Foundation for Parkinson's Research, ³Department of Medical and Molecular Genetics,Indiana University School of Medicine, ⁴Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital,University of Toronto, ⁵Division of Cell Signalling and Immunology, School of Life Sciences,University of Dundee, ⁶Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School,University of Dundee

Journal (Biochemistry)

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Filip Pamula^1,2, Jonas Mühle¹, Alain Blanc³, Rony Nehmé⁴, Patricia C. Edwards⁴, Christopher G. Tate⁴, Ching-Ju Tsai¹

¹Laboratory of Biomolecular Research,Paul Scherrer Institute, ²Department of Biology,ETH Zürich, ³Center for Radiopharmaceutical Sciences,Paul Scherrer Institute, ⁴Laboratory of Molecular Biology,Medical Research Council

Journal (Biochemistry)

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Hayley Abbiss^1,2,3, Joel P. A. Gummer^1,4, Michael Francki^5,6, Robert D. Trengove¹

¹Research and Innovation,Murdoch University, ²Centre for Digital Agriculture,Curtin University, ³Centre for Integrative Metabolomics and Computational Biology, School of Science,Edith Cowan University, ⁴Centre for Computational and System Medicine, Health Futures Institute,Murdoch University, ⁵Department of Primary Industries and Regional Development, ⁶State Agricultural Biotechnology Centre,Murdoch University

Journal (Biochemistry)

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

Damian Jacenik¹, Marta Zielińska², Sylwia Michlewska³, Jakub Fichna², Wanda M. Krajewska¹

¹Department of Cytobiochemistry, Faculty of Biology and Environmental Protection,University of Lodz, ²Department of Biochemistry, Faculty of Medicine,Medical University of Lodz, ³Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection,University of Lodz

Journal (Biochemistry)

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Emily E. Chea¹, Aimee Rinas², Jessica A. Espino¹, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences,University of Maryland Baltimore, ²AIT Bioscience

Journal (Biochemistry)

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

Manel Zeghal¹, Geneviève Laroche¹, Patrick M. Giguère^1,2

¹Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ²Brain and Mind Research Institute,University of Ottawa

Journal (Biochemistry)

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Justin R. Welden¹, Anna Pawluchin¹, Jacob van Doorn¹, Stefan Stamm¹

¹Institute for Molecular and Cellular Biochemistry,University of Kentucky

Journal (Biochemistry)

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Karamat Mohammad¹, Heng Jiang², Md. Israil Hossain¹, Vladimir I. Titorenko¹

¹Department of Biology,Concordia University, ²Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry,Concordia University

Journal (Biochemistry)

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Judith A. Stolwijk¹, Anne-Kathrin Mildner¹, Christian Kade¹, Michael Skiba¹, Guenther Bernhardt², Armin Buschauer², Harald Huebner³, Peter Gmeiner³, Joachim Wegener^1,4

¹Institute of Analytical Chemistry, Chemo- and Biosensors,University of Regensburg, ²Institute of Pharmacy,University of Regensburg, ³Department of Chemistry and Pharmacy,Friedrich-Alexander University Erlangen-Nuernberg, ⁴Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

Journal (Biochemistry)

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Sen Takeda¹, Kentaro Yoshimura¹, Hiroshi Tanihata²

¹Department of Anatomy and Cell Biology,University of Yamanashi School of Medicine, ²Shimadzu Corporation

Journal (Biochemistry)

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Yibing Deng¹, Bin Zheng¹, Yutong Liu¹, Shengchao Shi¹, Jingyuan Nie¹, Tao Wu¹, Peng Zheng¹

¹State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering,Nanjing University

Journal (Biochemistry)

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Jillian Rosenberg¹, Jun Huang^1,2

¹Committee on Cancer Biology,The University of Chicago, ²Pritzker School of Molecular Engineering,The University of Chicago

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