Connecticut Agricultural Experiment Station View Institution's Website 3 articles published in JoVE Neuroscience Base Recording: A Technique for Analyzing Responses of Taste Neurons in Drosophila Hany K. M. Dweck1,2, John R. Carlson1 1Department of Molecular, Cellular and Developmental Biology, Yale University, 2Department of Entomology, The Connecticut Agricultural Experiment Station A rarely used method of electrophysiological recording, base recording, allows analysis of features of taste coding that cannot be examined by conventional recording methods. Base recording also allows the analysis of taste responses to hydrophobic stimuli that cannot be studied using traditional electrophysiological methods. Immunology and Infection Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay Philip M. Armstrong1, Theodore G. Andreadis1, Shannon L. Finan1, John J. Shepard1, Michael C. Thomas1 1Center for Vector Biology and Zoonotic Diseases, Department of Environmental Sciences, The Connecticut Agricultural Experiment Station We describe a method to process and screen field-collected mosquitoes for a diversity of viruses by Vero cell culture assay. By employing this technique, we have detected 9 different viruses from 4 taxonomic families in mosquitoes collected in Connecticut. Immunology and Infection Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut Toru Kariu1, Adam S. Coleman1, John F. Anderson2, Utpal Pal1 1Department of Veterinary Medicine, University of Maryland, 2Department of Entomology, Connecticut Agricultural Experiment Station Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.
