Jerzy Kukuczka Academy of Physical Education 3 articles published in JoVE Biochemistry A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro Nikolai Fattakhov1, Silvia Torices1, Sarah Becker1, Timea Teglas1, Oandy Naranjo1, Michal Toborek1,2 1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, 2Institute of Physiotherapy and Health Sciences, The Jerzy Kukuczka Academy of Physical Education Here, we describe the method for establishing a triple cell culture model of the blood-brain barrier based on primary human brain microvascular endothelial cells, astrocytes, and pericytes. This multicellular model is suitable for studies of neurovascular unit dysfunction during ischemic stroke in vitro or for the screening of drug candidates. Medicine Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area Luc Bertrand1,2, Levi Dygert2, Michal Toborek1,3 1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, 2Miller School of Medicine, University of Miami, 3Jerzy Kukuczka Academy of Physical Education We describe a mouse model of stroke induced by the occlusion of the middle cerebral artery using a silicone coated suture. The protocol can be applied to induce permanent occlusion or a temporary ischemia, followed by reperfusion. Neuroscience Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery Ana R. Leda1, Levy Dygert1, Luc Bertrand1, Michal Toborek1,2 1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, 2The Jerzy Kukuczka Academy of Physical Education The present protocol describes a mouse microsurgery infusion technique, which effectively delivers substances directly into the brain via the internal carotid artery.