University of St Andrews View Institution's Website 5 articles published in JoVE Immunology and Infection A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) Wilber Sabiiti1, Bariki Mtafya1,2, Daniela Alferes De Lima1, Evelin Dombay1, Vincent O. Baron1, Khalide Azam3, Katarina Oravcova4, Derek J. Sloan1, Stephen H. Gillespie1 1Division of Infection and Global Health, School of Medicine, University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests. Neuroscience Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration Menghon Cheah1, James W. Fawcett1, Melissa R. Andrews2,3 1John van Geest Center for Brain Repair, University of Cambridge, 2School of Medicine, University of St. Andrews, 3Department of Biological Sciences, University of Southampton This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration. Chemistry Synthesis of Zeolites Using the ADOR (Assembly-Disassembly-Organization-Reassembly) Route Paul S. Wheatley1, Jiří Čejka2, Russell E. Morris1 1EaStChem School of Chemistry, The University of St Andrews, 2J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic A protocol for the preparation of novel zeolites by the ADOR (Assembly-Disassembly-Organization-Reassembly) synthetic route is presented. Medicine The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers Fiach C. O'Mahony1, Jyoti Nanda1, Alexander Laird1, Peter Mullen2, Helen Caldwell3, Ian M. Overton4, Lel Eory4, Marie O'Donnell1,5, Dana Faratian6, Thomas Powles7, David J. Harrison1,2, Grant D. Stewart1 1Edinburgh Urological Cancer Group, University of Edinburgh, 2School of Medicine, University of St Andrews, 3Division of Pathology, University of Edinburgh, 4MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, 5Department of Pathology, Western General Hospital, 6Breakthrough Breast Cancer Research Unit, University of Edinburgh, 7St Bartholomew's Cancer Institute, Experimental Cancer Medicine Centre, Queen Mary University of London RPPA enables the protein expression of hundreds of samples, printed on nitrocellulose slides to be interrogated simultaneously, using fluorescently labelled antibodies. This technique has been applied to study the effect of drug treatment heterogeneity within clear cell renal carcinoma. Engineering Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities Christopher Paul Reardon1, Isabella H. Rey1, Karl Welna1, Liam O'Faolain1, Thomas F. Krauss1 1School of Physics & Astronomy, University of St Andrews Use of photonic crystal slow light waveguides and cavities has been widely adopted by the photonics community in many differing applications. Therefore fabrication and characterization of these devices are of great interest. This paper outlines our fabrication technique and two optical characterization methods, namely: interferometric (waveguides) and resonant scattering (cavities).