Northwest University 4 articles published in JoVE Biology Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues Yuan Cao*1,2, Ji-Yu Qiu*1,3, Da Chen1,2, Chen-Yao Li1,4, Shu-Juan Xing1,5, Chen-Xi Zheng1, Xin Liu2, Yan Jin1,6, Bing-Dong Sui1 1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6 The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos. Environment A Complete Method for Evaluating the Performance of Photocatalysts for the Degradation of Antibiotics in Environmental Remediation Bing Wang1, XuXia Zhang1, LeCheng Li1, MengTing Ji1, Zheng Zheng1, ChuanHui Shi1, Zhuo Li1, Hong Hao1 1School of Chemical Engineering, Northwest University Presented here is a protocol to explore a universal set of experimental procedures for comprehensive laboratory evaluation of photocatalysts in the field of environmental purification, using the example of photocatalytic removal of antibiotic organic pollutant molecules from water by phthalocyanine sensitized silver phosphate composites. Biology Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells Shu-Juan Xing1,2, Kai-Chao Zhang2,3, Si-Yuan Tang2,4, Lu Liu2,5, Yuan Cao2,5, Chen-Xi Zheng2, Bing-Dong Sui2, Yan Jin2,3 1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3 The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article. Bioengineering Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device Bingchen Che*1, Jie Zhu*1, Dan Sun1, Xiaoqiang Feng1, Ce Zhang1 1State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology, Northwest University We present a microfluidic system for high throughput studies on complex life machinery, which consists of 1500 culture units, an array of enhanced peristaltic pumps and an on-site mixing modulus. The microfluidic chip allows for the analysis of the highly complex and dynamic micro-environmental conditions in vivo.