King Faisal Specialty Hospital and Research Center 1 article published in JoVE Bioengineering Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves Sharan Ramaswamy1, Manuel Salinas1, Rob Carrol2, Karla Landaburo1, Xavier Ryans1, Cynthia Crespo1, Ailyn Rivero1, Faris Al-Mousily3,4, Curt DeGroff3, Mark Bleiweis3, Hitomi Yamaguchi2 1Tissue Engineered Mechanics, Imaging and Materials Laboratory, Department of Biomedical Engineering, Florida International University, 2Department of Mechanical and Aerospace Engineering, University of Florida, 3College of Medicine, University of Florida, 4King Faisal Specialty Hospital and Research Center, Jeddah, Saudi Arabia There has been renewed interest in developing polymer valves. Here, the objectives are to demonstrate the feasibility of modifying a commercial pulse duplicator to accommodate tri-leaflet geometries and to define a protocol to present polymer valve hydrodynamic data in comparison to native and prosthetic valve data collected under near-identical conditions.