JOVE JOURNAL

Biochimica

Showing 181 - 192 in 776 videos.

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Journal (Biochemistry)

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Eliana M. Wolpaw*¹, Marissa L. Frenett*¹, Claudia A. Mak¹, Sloane M. Zwanger¹, Madushi Raththagala¹

¹Department of Chemistry,Skidmore College

Journal (Biochemistry)

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Artem Bonchuk^1,2, Nikolay Zolotarev¹, Konstantin Balagurov^1,2, Olga Arkova², Pavel Georgiev¹

¹Department of the Control of Genetic Processes, Institute of Gene Biology,Russian Academy of Sciences, ²Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology,Russian Academy of Sciences

Journal (Biochemistry)

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Sarah-Luisa Dülk¹, Silvia Rouskin¹

¹Department of Microbiology,Harvard Medical School

Journal (Biochemistry)

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Sunday Solomon Josiah¹, Nur Farah Meor Azlan¹, Asami Oguro-Ando¹, Jinwei Zhang¹

¹Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences,University of Exeter, Hatherly Laboratories

Journal (Biochemistry)

A “Dual-Addition” Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

Caixing Xiong¹, Dwight Baker², Patricia Pietrantonio¹

¹Department of Entomology,Texas A&M University, ²Department of Biochemistry and Biophysics,Texas A&M University

Journal (Biochemistry)

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

Xinwen Miao*¹, Jiawei Wu*^1,2, Wenting Zhao^1,3

¹School of Chemistry, Chemical Engineering, and Biotechnology,Nanyang Technological University, ²State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering,Zhejiang University of Technology, ³Institute for Digital Molecular Analytics and Science,Nanyang Technological University

Journal (Biochemistry)

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Jonathan T. Specker¹, Alexander B. Smith², Orlaith Keenan², Joseph P. Zackular^2,3, Boone M. Prentice¹

¹Department of Chemistry,University of Florida, ²Division of Protective Immunity,Children’s Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine, Perelman School of Medicine,University of Pennsylvania

Journal (Biochemistry)

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids

Julia Maldonado-Valderrama¹, Teresa del Castillo Santaella², Juan Antonio Holgado-Terriza³, Miguel Ángel Cabrerizo-Vílchez¹

¹Department of Applied Physics, Faculty of Sciences, Campus de Fuentenueva,University of Granada, ²Department of Physical Chemistry, Faculty of Pharmacy, Campus de Cartuja,University of Granada, ³Department of Software Engineering, School of Computer and Telecommunication Engineering, Campus de Aynadamar,University of Granada

Journal (Biochemistry)

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Piero R. Bianco¹

¹Department of Pharmaceutical Sciences, College of Pharmacy,University of Nebraska Medical Center

Journal (Biochemistry)

Shotgun Lipidomics of Rodent Tissues

Oksana Lavrynenko¹, Sophie Dijon¹, Björn Titz¹, Nikolai V. Ivanov¹

¹PMI R&D,Philip Morris Products SA

Journal (Biochemistry)

Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

Kristen L. Segars*¹, Nicholas A. Azzari*², Stephanie Gomez², Celeste B. Rich³, Vickery Trinkaus-Randall^2,3

¹Department of Pharmacology, School of Medicine,Boston University, ²Department of Biochemistry, School of Medicine,Boston University, ³Department of Ophthalmology, School of Medicine,Boston University

Journal (Biochemistry)

A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

Nikolai Fattakhov¹, Silvia Torices¹, Sarah Becker¹, Timea Teglas¹, Oandy Naranjo¹, Michal Toborek^1,2

¹Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, ²Institute of Physiotherapy and Health Sciences,The Jerzy Kukuczka Academy of Physical Education

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