Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Kathryn M. Crotty; Samantha M. Yeligar

doi:10.3791/67579

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Immunologia e infezioni

Ethanol Impairs AM Bioenergetics by Shifting to Glutamine-Dependent Respiration

November 15, 2024

Author Spotlight: Investigating the Impact of Chronic Alcohol Misuse on Alveolar Macrophage Immune Function and Mitochondrial Metabolism

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Kathryn M. Crotty^1,2, Samantha M. Yeligar^1,2

¹Department of Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine,Emory University, ²Atlanta Veterans Affairs Health Care System

Summary

Alcohol misuse impairs alveolar macrophage (AM) immunity due to suppressed mitochondrial respiration and bioenergetics. We recently demonstrated that ethanol (EtOH) exposure increases glutamine dependency for mitochondrial respiration in AMs. Herein, methods are provided to determine the usage of glutamine for mitochondrial respiration in EtOH-treated AMs using an extracellular flux bioanalyzer.

Ethanol Impairs AM Bioenergetics by Shifting to Glutamine-Dependent Respiration

Author Spotlight: Investigating the Impact of Chronic Alcohol Misuse on Alveolar Macrophage Immune Function and Mitochondrial Metabolism

Summary

Article