Isolating Human Peripheral Blood Mononuclear Cells from Buffy Coats <i>via </i> High Throughput Immunomagnetic Bead Separation

Maria Villalva; Sara Macphail; Yingshi Li; Beth Caruana

doi:10.3791/66887

High-Throughput PBMC Isolation Using Immunomagnetic Beads

July 19, 2024

Isolating Human Peripheral Blood Mononuclear Cells from Buffy Coats via High Throughput Immunomagnetic Bead Separation

00:00 min

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Maria Villalva, Sara Macphail, Yingshi Li, Beth Caruana

¹New South Wales Health Statewide Biobank,New South Wales Health Pathology

Summary

This protocol details a high-throughput automation-compatible method to isolate human peripheral blood mononuclear cells for biobanking and other purposes.

High-Throughput PBMC Isolation Using Immunomagnetic Beads

Isolating Human Peripheral Blood Mononuclear Cells from Buffy Coats via High Throughput Immunomagnetic Bead Separation

Summary

Article