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Flow Cytometry-Based Assays for Microglial Engulfment of Synaptic Materials

Flow Cytometry-Based Assays for Microglial Engulfment of Synaptic Materials

May 31, 2024

Author Spotlight: Advancing Understanding Through Technological Innovations in Psychoneuroimmunology

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In Vivo Quantification of Microglial Engulfment of Glutamatergic Synapses with Intracellular vGLUT1 Staining

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Flow Cytometric Quantification of In Vitro Microglial Engulfment of Crude Synaptosomes for the Analysis of Synaptic Refinement

03:15 min
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1Psychoneuroimmunology,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, 2Experimental Ophthalmology,Charité Universitätsmedizin Berlin

Summary

Here we present two protocols to quantify microglial engulfment of vGLUT1-positive synapses and pHRodo Red-labeled crude synaptosomes using flow cytometry.

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