Optimized Whole Blood Immunoassays for Analyzing Fungal and Viral Immunity
1Institute for Laboratory Medicine and Microbiology,University Hospital Augsburg, 2Department of Internal Medicine II,University Hospital of Wuerzburg, 3Internal Medicine III – Gastroenterology and Infectious Diseases,University Hospital Augsburg, 4Department of Infectious Diseases, Infection Control and Employee Health, MD Anderson Cancer Center,University of Texas Houston
Summary
Whole blood-based immunoassays provide a facile and resource-efficient tool to analyze antigen-specific immunity for diagnostic and research purposes. This article provides an optimized whole blood-based protocol with dual co-stimulation for comprehensive analysis of host immunity to fungal and viral pathogens, including a low-volume version for pediatric patients and small animals.
