Light-Controlled Reversible Protein Patterning on Lipid Membranes

February 23, 2024

模型膜上的动态光诱导蛋白质模式

mOrange-Nano Recruitment to disiLID Functionalized Supported Lipid Bilayers (SLBs) for Generating Protein Patterns

Preparation of Giant Unilamellar Vesicles (GUVs) for Fabricating Light-Regulated Reversible Protein Patterns

mOrange-Nano Recruitment to disiLID Functionalized Giant Unilamellar Vesicles (GUVs) for Generating Protein Patterns

Daniele Di Iorio, Seraphine V. Wegner

¹Institute of Physiological Chemistry and Pathobiochemistry,University of Münster

Summary

在这里，描述了一种协议，用于在人工脂质膜上以高时空精度生成光调节和可逆的蛋白质模式。该方法包括固定在模型膜上的蛋白质 iLID（改进的光诱导二聚体）的局部光激活，该模型膜在蓝光下与其伙伴蛋白 Nano（野生型 SspB）结合。

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