Measurement of Poly A Tail Length from <em>Drosophila</em> Larva Brain and Cell Line

Monika Singh; Jung Hwan Kim

doi:10.3791/66116

Rivista

Neuroscienze

Quantifying the Poly(A) Length of Genes from the Drosophila Nervous System

Quantifying the Poly(A) Length of Genes from the Drosophila Nervous System

January 12, 2024

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques

01:21 min

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Isolation of Brain from Drosophila Larvae and Harvest of S2 Schneider Cells

01:43 min

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Total RNA Extraction from Drosophila Larvae Brain and S2 Schneider Cells

01:51 min

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Poly A Tail Length Measurement of Genes from Drosophila Brain

03:19 min

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42 Views

Monika Singh¹, Jung Hwan Kim¹

¹Department of Biology,University of Nevada, Reno

Summary

The protocol describes an efficient and reliable method for quantifying the poly(A) length of the gene of interest from the Drosophila nervous system, which can be easily adapted to tissues or cell types from other species.