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Biochimica
Coating CryoEM Grids with Monolayer Graphene to Improve Cryo-Sample Preparation

Coating CryoEM Grids with Monolayer Graphene to Improve Cryo-Sample Preparation

November 10, 2023

Author Spotlight: Enhancing CryoEM Sample Preparation Using Graphene Monolayer on Microscopy Grids

01:38 min
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865 Views

Preparing MMA-Coated CVD Graphene for Fabrication of Graphene-Coated CryoEM Grids

01:54 min
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147 Views

Preparing Grid-Sized MMA-Coated CVD Graphene Squares for Attaching to CryoEM Grids

01:49 min
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60 Views

Fabricating Graphene-Coated Cryo-EM Grids Using Grid-Sized MMA-Coated CVD Graphene Squares

02:43 min
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128 Views
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1Department of Biology,Massachusetts Institute of Technology, 2Program in Computational and Systems Biology,Massachusetts Institute of Technology

Summary

The application of support layers to cryogenic electron microscopy (cryoEM) grids can increase particle density, limit interactions with the air-water interface, reduce beam-induced motion, and improve the distribution of particle orientations. This paper describes a robust protocol for coating cryoEM grids with a monolayer of graphene for improved cryo-sample preparation.

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