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Biochimica
Automated Protein Crystallization and Real-Time Data Collection

Automated Protein Crystallization and Real-Time Data Collection

March 08, 2024

Author Spotlight: Advancing Protein Structure Analysis for Drug Development

00:48 min
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In Situ X-ray Crystallographic Data Collection Using the Beamline at Diamond Light Source

06:21 min
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1Diamond Light Source Ltd,Harwell Science and Innovation Campus, 2Research Complex at Harwell,Harwell Science and Innovation Campus

Summary

We present a protocol for the crystallization of proteins using the crystallization facility in the Research Complex at Harwell and subsequent in situ X-ray crystallographic data collection from crystals within the plates at Diamond's Versatile Macromolecular Crystallography in situ (VMXi) beamline. We describe sample requirements, crystallization protocols, and data collection guidelines.

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