Grafting Human Brain Organoids into the Chorioallantoic Membrane of the Chick Embryo
1Instituto de Biología Celular y Neurociencias “Prof. E. De Robertis” (IBCN),CONICET – Universidad de Buenos Aires, 2Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética,Universidad de Buenos Aires, 3Institute of Neurosciences, Pathology and Experimental Therapeutics Dept,University of Barcelona, 4Functional Neurogenomics Group, Neurodevelopmental Disorders,IDIBELL, L’Hospitalet de Llobregat, 5IBE, Institute of Evolutionary Biology (UPF-CSIC), Department of Medicine and Life Sciences,Universitat Pompeu Fabra, 6Institució Catalana de Recerca i Estudis Avançats (ICREA) and Universitat Pompeu Fabra, 7Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, 8BarcelonaBeta Brain Research Center,Pasqual Maragall Foundation, 9Instituto de Investigación en Biomedicina (IBioBA) – CONICET – Instituto Partner de la Sociedad Max Planck
Summary
Here, we present a protocol to engraft human brain organoids at multiple maturation stages into the chick chorioallantoic membrane (CAM). Brain organoids were grown following unguided standardized protocols.
