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Isolating Small Extracellular Vesicles and Extracting Their Peptidome

Isolating Small Extracellular Vesicles and Extracting Their Peptidome

June 30, 2023

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

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Isolating Small Extracellular Vesicles (SEVs) by Differential Ultracentrifugation

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Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs)

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1School of Basic Medical Sciences,Anhui Medical University, 2State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, 3School of Life Sciences,Hebei University

Summary

This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry.

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