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Biologia
Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

June 02, 2023

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

02:28 min
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1,083 Views

Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection

02:33 min
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152 Views

Semiautomated Analysis of mtHyPer7 in Yeast Cells with Scripts

05:11 min
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128 Views
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1Department of Pathology and Cell Biology,Columbia University Irving Medical Center, 2Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center,Columbia University Irving Medical Center

Summary

Hydrogen peroxide (H2O2) is both a source of oxidative damage and a signaling molecule. This protocol describes how to measure mitochondrial H2O2 using mitochondria-targeted HyPer7 (mtHyPer7), a genetically encoded ratiometric biosensor, in living yeast. It details how to optimize imaging conditions and perform quantitative cellular and subcellular analysis using freely available software.

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