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Identifying G-Quadruplexes in a DNA Template Using Chemical Mapping

Identifying G-Quadruplexes in a DNA Template Using Chemical Mapping

May 12, 2023

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

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Folding and Probing of Nucleic Acid Constructs

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Separation of Polymerized Nucleic Acid Constructs by PAGE

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1Department of Pharmaceutical and Pharmacological Sciences,University of Padova, 2Institute of Organic Chemistry,Justus Liebig University Giessen

Summary

Bis-3-chloropiperidines (B-CePs) are useful chemical probes to identify and characterize G-quadruplex structures in DNA templates in vitro. This protocol details the procedure to perform probing reactions with B-CePs and to resolve reaction products by high-resolution polyacrylamide gel electrophoresis.

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