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A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages

A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages

August 04, 2023

Author Spotlight: Efficient CRISPR/Cas9 Genome Editing in Bone Marrow-Derived Macrophages for Precise Gene Disruption

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Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages

04:03 min
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1Department of Internal Medicine,University of California, Davis, 2Department of Medical Microbiology and Immunology,University of California, Davis

Summary

This protocol describes the procedure for genome editing in mouse bone marrow-derived macrophages using Cas9-sgRNA ribonucleoprotein complexes assembled in vitro and delivered by electroporation.

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