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1Department of Molecular Biosciences,The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action,Michigan State University, 3Department of Microbiology and Molecular Genetics,Michigan State University, 4Department of Integrative Biology,Michigan State University, 5Biological Sciences Program,Michigan State University
Chapitres
- 00:03Introduction
- 02:02Daily Transfers of LTEE Populations
- 06:04Competitive Fitness Assays
- 06:44Prepare Supplies
- 08:24Day -2: Revive Competitors
- 09:09Day -1: Precondition Competitors
- 09:47Day 0: Begin Competition
- 11:16Alternative:Continue Three-Day Competition
- 11:43Day 1 or 3: Finish Competition
- 12:41Calculate and Plot Fitness
- 12:52Representative Results Appearance and turbidity of LTEE Cultures
- 13:22Representative Results Co-culture Competition Results
- 14:39Conclusion
Este protocolo descreve como manter o Experimento de Evolução de Longo Prazo (LTEE) de Escherichia coli realizando suas transferências diárias e congelamentos periódicos e como conduzir ensaios de competição para medir melhorias de aptidão em bactérias evoluídas. Esses procedimentos podem servir como um modelo para pesquisadores que iniciam seus próprios experimentos de evolução microbiana.
