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Immunologie et infection

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Primer Murin T-hücrelerinde T-hücresi Reseptörü Kaynaklı Kalsiyum Akışının Tam Spektrumlu Akış Sitometrisi ile Analizi

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Immunologie et infection

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Journal JoVE Immunologie et infection

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

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Primer Murin T-hücrelerinde T-hücresi Reseptörü Kaynaklı Kalsiyum Akışının Tam Spektrumlu Akış Sitometrisi ile Analizi

3,173 Views

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10:01 min

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December 16, 2022

DOI:

10.3791/64526-v

DOI:

10.3791/64526-v

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10:01 min

December 16, 2022

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12 Views

Loni Perrenoud, James Conley, Leslie J. Berg

¹Department of Immunology and Microbiology,University of Colorado-Anschutz School of Medicine

Tags

T-cell Receptor Calcium Influx Flow Cytometry Indo-1 AM Cell Staining Cell Phenotyping Cell Subsets EGTA Ionomycin Fc Receptor Blocking Cell Washing Master Mix Antibody Staining

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