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全谱流式细胞术分析原代小鼠T细胞受体诱导的钙内流
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Immunologie et infection
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Journal JoVE Immunologie et infection
Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry
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全谱流式细胞术分析原代小鼠T细胞受体诱导的钙内流

3,173 Views

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10:01 min

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December 16, 2022

DOI:

10.3791/64526-v

DOI:
10.3791/64526-v

•

10:01 min
December 16, 2022

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12 Views
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1Department of Immunology and Microbiology,University of Colorado-Anschutz School of Medicine

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T-cell ReceptorCalcium InfluxFlow CytometryIndo-1 AMCell StainingCell PhenotypingCell SubsetsEGTAIonomycinFc Receptor BlockingCell WashingMaster Mix Antibody Staining

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Perrenoud, L., Conley, J., Berg, L. J. Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry. J. Vis. Exp. (190), e64526, doi:10.3791/64526 (2022).

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