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Journal
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Neurosciences
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Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
Journal JoVE
Neurosciences
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Journal JoVE
Neurosciences
Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
DOI:
10.3791/64420-v
•
05:27 min
•
September 28, 2022
•
Peter A. Santi
,
Shane B. Johnson
1
Department of Otolaryngology
,
University of Minnesota
Chapitres
00:04
Introduction
00:43
Cochlea Removal and Tissue Processing
02:39
Imaging of Cochleae
03:41
Results: Imaging of Cochlea and Analysis of SGN Density Along the Rosenthal s Canal
04:46
Conclusion
Summary
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A light-sheet microscope was developed to image and digitize whole cochlea.
Tags
Keywords Extracted From The Given Text:Light-sheet Fluorescence Microscopy
Cochlea
Aging
Hair Cells
Spiral Ganglion Neurons
Mouse
Temporal Bone
Fixation
Formalin
Decalcification
EDTA
Dehydration
Ethanol
Rhodamine B
Spalteholz Solution
Clearing Solution
Article
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