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08:31 min
November 04, 2022
DOI:
10.3791/64373-v
在这里,我们描述了一种新的流式细胞术方法,用于直接从新鲜小鼠骨髓和脾脏中前瞻性分离早期爆发形成单位红系(BFU-e)和集落形成单位红系(CFU-e)祖细胞。该协议基于单细胞转录组学数据开发,是第一个以高纯度分离所有组织红系祖细胞的方案。
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Swaminathan, A., Hwang, Y., Winward, A., Socolovsky, M. Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry. J. Vis. Exp. (189), e64373, doi:10.3791/64373 (2022).
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