Journal
/
Neurosciences
/
Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

DOI:
10.3791/64323-v

•

11:19 min

•

August 18, 2022

•

,
1Department of Neurology,University of Massachusetts Chan Medical School, 2Translation Science Program, Morningside Graduate School of Biomedical Sciences,University of Massachusetts Chan Medical School

Chapitres

  • 00:04Introduction
  • 00:44Embryoid Body (EB) Formation
  • 04:20Live‐Cell Phagocytosis Assay
  • 05:48Imaging Acquisition and Analysis
  • 08:42Results: Generating Human Microglia‐Like Cells and Synaptosomes for In Vitro Live‐Cell Phagocytosis Assay
  • 10:50Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes the differentiation process of human induced pluripotent stem cells (iPSCs) into microglia-like cells for in vitro experimentation. We also include a detailed procedure for generating human synaptosomes from iPSC-derived lower motor neurons that can be used as a substrate for in vitro phagocytosis assays using live-cell imaging systems.

Tags

IPSC-derived MicrogliaPhagocytosis AssayHuman MicrogliaMicroglia DifferentiationPrimitive Macrophage PrecursorsMatrigel CoatingEB Differentiation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Read Article