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Deep Fluorescence Observation in Rice Shoots via Clearing Technology
 

Deep Fluorescence Observation in Rice Shoots via Clearing Technology

Article DOI: 10.3791/64116-v • 07:21 min • June 27th, 2022
June 27th, 2022 •
1, 2, 2, 3,4
1Graduate School of Bioagricultural Sciences, Nagoya University, 2Bioscience and Biotechnology Center, Nagoya University, 3Institute for Advanced Research (IAR), Nagoya University, 4Institute of Transformative Bio-Molecules (ITbM), Nagoya University

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The present protocol describes a clearing technique for rice shoots, which are difficult to prepare for internal structural observations owing to the hard, thick, or layered nature of the tissues. This method facilitates continuous and deep fluorescence observations, even in adult rice plants.

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Deep Fluorescence Observation Rice Shoots Clearing Technology Tissue Penetration Confocal Microscope Structure Observation Macro Perspective Hard And Thick Tissues Adult-erized Shoots Deep Imaging Plant Species Plant Biological Research Roots Tweezers Outer Leaves Single-edged Razor Shoot Apical Meristem Young Panicle Oval Cross Section Double-edged Razor Fixative Solution Paraffin Film
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